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Human Anti-Sperm Antibody (Anti-AsAb) ELISA Kit

SKU: orb781908
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Description

The Human Anti-Sperm Antibody (Anti-AsAb) ELISA Kit uses a competitive inhibition enzyme immunoassay technique to quantify Anti-Sperm Antibody (Anti-AsAb) in human serum, plasma and other biological fluids. The kit has a detection range of 3.13-200 ng/mL and a sensitivity of 1.19 ng/mL.

Research Area

Stem Cell & Developmental Biology

Images & Validation

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Tested ApplicationsELISA
Application Notes
standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-AsAb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-AsAb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-AsAb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-AsAb in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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ReactivityHuman
Sample Typesserum, plasma and other biological fluids
Assay TypeSandwich
Assay Time3.5h
Range3.13-200 ng/mL
Sensitivity1.19 ng/mL
Concentration200 ng/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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Anti-Spermatozoa Antibodies ELISA Kit, Sperm Antibodies ELISA Kit, Antisperm Antibodies ELISA Kit
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Available Sizes

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48 T
$ 330.00
96 T
$ 470.00
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