Human Aromatase (ARO) ELISA Kit

SKU: orb776759

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aromatase(ARO).

Research Area

Cancer Biology, Metabolism Research, Protein Biochemistry

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ARO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ARO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ARO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ARO in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.059 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CYP19A1 ELISA Kit, CYPXIX ELISA Kit, ARO ELISA Kit, ARO1 ELISA Kit, CPV1 ELISA Kit, CYAR ELISA Kit, CYP19 ELISA Kit, P450AROM ELISA Kit, P450-AROM ELISA Kit, Estrogen synthase ELISA Kit, Cytochrome P450,Family 19,Subfamily A,Polypeptide 1 ELISA Kit

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Quick Database Links

UniProt

P11511

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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