Human B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit

SKU: orb774971

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Lymphocyte Chemoattractant 1(BLC1).

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Tested Applications

ELISA

Application Notes

standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BLC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BLC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BLC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BLC1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	15.63-1000 pg/mL
Sensitivity	6.2 pg/mL
Concentration	1000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CXCL13 ELISA Kit, SCYB13 ELISA Kit, BLC ELISA Kit, BCA1 ELISA Kit, BLR1L ELISA Kit, ANGIE ELISA Kit, ANGIE2 ELISA Kit, Chemokine(C-X-C-Motif)ligand 13 ELISA Kit, Small Inducible Cytokine B Subfamily(Cys-X-Cys Motif)Member 13 ELISA Kit

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Human B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Human (1)

B Gajewski, I Karlińska, M Domowicz, I Bednarski No Relation Between Cognitive Impairment, Physical Disability and Serum Biomarkers in a Cohort of Progressive Multiple Sclerosis Patients Biomolecules, (2025)

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Applications

ELISA

Reactivity

Human