Human B3GNT1 protein

SKU: orb391276

Description

Recombinant human B3GNT1 protein is produced by our mammalian expression system and the target gene encoding Asp43-Cys415 is expressed with a 6His tag at the C-terminus.

Research Area

Epigenetics

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	Human cells
Reactivity	Human
Protein Sequence	DQYFEFFPPS PRSVDQVKAQ LRTALASGGV LDASGDYRVY RGLLKTTMDP NDVILATHAS VDNLLHLSGL LERWEGPLSV SVFAATKEEA QLATVLAYAL SSHCPDMRAR VAMHLVCPSR YEAAVPDPRE PGEFALLRSC QEVFDKLARV AQPGINYALG TNVSYPNNLL RNLAREGANY ALVIDVDMVP SEGLWRGLRE MLDQSNQWGG TALVVPAFEI RRARRMPMNK NELVQLYQVG EVRPFYYGLC TPCQAPTNYS RWVNLPEESL LRPAYVVPWQ DPWEPFYVAG GKVPTFDERF RQYGFNRISQ ACELHVAGFD FEVLNEGFLV HKGFKEALKF HPQKEAENQH NKILYRQFKQ ELKAKYPNSP RRCVDHHHHH H
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

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Storage	Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/Appearance	Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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B3GNT6; N-acetyllactosaminide beta-13-N-acetylglucosaminyltransferase; I-beta-13-N-acetylglucosaminyltransferase; iGnT; Poly-N-acetyllactosamine extension enzyme; UDP-GlcNAc:betaGal beta-13-N-acetylglucosaminyltransferase 1

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Quick Database Links

UniProt

O43505

NCBI Gene ID

11041

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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