Human B7-2 Protein, mFc-His Tag

SKU: orb689395

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Description

Recombinant human B7-2 protein with C-terminal mouse Fc and 6×His tag

Images & Validation

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Item 1 of 4

Key Properties

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Source	Mammalian
Target	B7-2
Reactivity	Human
Tag	C-Mouse Fc and 6×His Tag
Molecular Weight	The protein has a predicted molecular mass of 52.4 kDa after removal of the signal peptide.
Purification	Purified from cell culture supernatant by affinity chromatography
Purity	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

Storage & Handling

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Storage	Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Form/Appearance	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Buffer/Preservatives	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Disclaimer	For research use only

Alternative Names

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CD86;B7-2;B70;CD28LG2;LAB72;MGC34413

Quality Guarantee

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Human PD-L1 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human PD-L1, mFc-His tagged protein orb689384 can bind Anti-PDL1 Neutralizing antibody orb689314 in a linear range of 0.24-7.81 ng/ml.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human PD1, hFc-His tagged protein orb689448 can bind Human PDL1, mFc-His tagged protein (orb689384) in a linear range of 62.5-251.1 ng/ml.

Flow cytometry analysis with 15 μg/mL Human PD-L1 Protein, mFc-His tag (orb689384) on Expi293 cells transfected with human PD1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Quick Database Links

Gene Symbol

B7-2

UniProt

P42081

UniProt Details

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No UniProt data available

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