Human Bradykinin (BK) ELISA Kit

SKU: orb1807119

Featured

Description

Human Bradykinin (BK) ELISA Kit

Research Area

Cancer Biology, Immunology & Inflammation, Infectious Disease & Virology

Images & Validation

−

Item 1 of 1

Application Notes

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human BK. During the reaction, Human BK in the sample or standard competes with a fixed amount of Human BK on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human BK. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human BK in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Key Properties

−

Reactivity	Human
Sample Types	serum, plasma, Tissue homogenate and Other biological samples
Assay Type	Competitive
Assay Time	1.5H
Range	1.56-100ng/mL
Sensitivity	0.59 ng/mL

Storage & Handling

−

Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

−

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Quick Database Links

Gene Symbol

UniProt

P01042

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Manual

Product Manual

Download

Request a Document

Protocol Information

Find More Protocols