Human Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) ELISA Kit

SKU: orb777556

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Breast Cancer Anti-Estrogen Resistance 1(BCAR1).

Research Area

Cancer Biology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BCAR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BCAR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BCAR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BCAR1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma, tissue homogenates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	78.13-5000 pg/mL
Sensitivity	26 pg/mL
Concentration	5000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CAS ELISA Kit, CRKAS ELISA Kit, P130Cas ELISA Kit, CASS1 ELISA Kit, Crk-Associated Substrate ELISA Kit, Cas Scaffolding Protein Family Member 1 ELISA Kit

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Human Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) ELISA Kit

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UniProt

P56945

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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