Human C4BPb(C4 Binding Protein Beta) Microsample ELISA Kit

SKU: orb2998905

Description

Research Area

Immunology & Inflammation, Infectious Disease & Virology, Stem Cell & Developmental Biology

Images & Validation

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Application Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C4BPb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C4BPb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C4BPb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C4BPb in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	31.25-2000 pg/mL
Sensitivity	14.1 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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C4BP-B; C4-BP; Complement Component 4 Binding Protein

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Quick Database Links

UniProt

P20851

UniProt Details

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No UniProt data available

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