Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1
Clone No.	4H11
Immunogen	KLH conjugated synthetic peptide derived from human CD34
Target	CD34
Molecular Weight	105-120 kDa
Purification	Affinity purified by Protein G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4).
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD34_HUMAN; CD34;

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Blank control: TF-1. Primary Antibody (green line): Mouse Anti-human CD34 antibody (orb526618), dilution: 0.5 ug/Test, Secondary Antibody (white blue line): Goat anti-mouse IgG-FITC, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Mouse IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (human placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (human CD34) Monoclonal Antibody, Unconjugated (orb526618) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Quick Database Links

Gene Symbol

CD34

UniProt

P28906

UniProt Details

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No UniProt data available

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Protocol Information

Flow Cytometry

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