Human Chloride Intracellular Channel Protein 1 (CLIC1) ELISA Kit

SKU: orb777707

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chloride Intracellular Channel Protein 1(CLIC1).

Research Area

Metabolism Research, Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CLIC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CLIC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CLIC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CLIC1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.117 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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G6 ELISA Kit, NCC27 ELISA Kit, p64CLCP ELISA Kit, hRNCC ELISA Kit, Chloride channel ABP ELISA Kit, Nuclear chloride ion channel 27 ELISA Kit, Regulatory nuclear chloride ion channel protein ELISA Kit

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Human Chloride Intracellular Channel Protein 1 (CLIC1) ELISA Kit

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UniProt

O00299

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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