Human Collagen Type I protein

Glycated collagen 1 regulates adipocyte insulin signaling and Rho and AGE scavenger receptor expression. Preadipocytes from human VAT were differentiated into mature adipocytes in 2D culture + /− unglycated or glycated recombinant human collagen 1 (UC1, GC1), then studied with glucose uptake assay or RT-qPCR. (a) Glucose uptake in non-stimulated (basal) or insulin-stimulated conditions. Ordinate: mean glucose uptake measured by 3H-2D-glucose in cell lysates (cpm) normalized to cell lysate protein concentration (mg/ml); bars with different letters indicate P < 0.050; n = 15 NDM, 15 DM subjects. (b) Gene expression studied with RT-qPCR. Ordinate: mean fold difference in transcript level in GC1 arm relative to UC1 arm referent = 1; *P < 0.050, comparing transcript levels in GC1 arm vs. UC1 arm; n = 12 NDM, 12 DM subjects.

SDS-PAGE Results of Human Collagen Type I. Lane 1: Human Collagen Type I Reduced (5.0 µg). Lane 2: Opal PRestained Molecular Weight Marker. Lane 3: Human Collagen Type I Non-Reduced (5.0 µg). 7.5% Gel, Coomassie Stained. Observed MW: Type I trimer, Type I dimer α1, α2, Type I monomer α1, α2.

Western blot of Human Collagen Type I. Lane 1: Human Collagen Type 1. Load: 50 ng per lane. Primary antibody: Collagen Type I antibody at 1:1000 overnight at 4°C. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 139 & 130 kDa, 139 & 130 kDa for Collagen Type I. Other Band(s): Collagen Type I splice variants and isoforms.