Human Collagen Type III protein

SKU: orb750383

Description

Research Area

Protein Biochemistry

Images & Validation

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Item 1 of 2

Tested Applications

ELISA, IHC, IP, SDS-PAGE, WB

Application Notes

Human Collagen Type III purified protein standard is used as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation, and for other immunological assays as optimized by the user. Specific conditions should be optimized by user. Collagen Type III is recognized by type specific anti-collagen antibodies that recognize a native three-dimensional structure.

Key Properties

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Source	Human
Biological Origin	Human
Target	COL3A1
Purity	Human Collagen Type III has been prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with Anti-Collagen Type III. Reaction with Anti-Collagen I, II, IV, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).
Conjugation	Unconjugated

Storage & Handling

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Storage	Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.5 M Acetic Acid
Concentration	1.0 mg/mL
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Type III collagen protein, collagen 3 protein, human collagen protein, Alpha 1 type III collagen protein, Alpha1 (III) collagen protein, COL3A1_HUMAN protein, Collagen Type III Alpha 1 Chain protein, Ehlers-Danlos Syndrome Type IV Autosomal Dominant protein, Fetal Collagen protein, Human placenta Collagen Type III Protein protein, Human Type III Collagen Protein protein, scleroprotein collagen protein

Quality Guarantee

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SDS-PAGE Results of Human Collagen Type III. Lane 1: Human Collagen Type III Reduced [1.0 µg]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Human Collagen Type III Non-Reduced [1.0 µg]. 4-20% Gel Coomassie Stained. Observed: α1 bands.

Western Blot of COLLAGEN III. Lane 1: Human Collagen III. Load: 100 ng per lane. Primary antibody: Collagen III Antibody at 1:1000 o/n at 4°C. Secondary antibody: DyLight™ 649 Goat anti-rabbit at 1:20000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 138 kDa, 138 kDa.

Quick Database Links

Gene Symbol

COL3A1

UniProt

P02461

RefSeq (Protein):NP_000081.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_000081.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Immunoprecipitation

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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