Human Complement C5 Protein, Fc Tag

SKU: orb1806482

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Description

Human Complement C5 Protein, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Cys 1676 (Accession # P01031-1).

Research Area

Immunology & Inflammation

Images & Validation

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Application Notes

This protein carries a human IgG1 Fc tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.9 kDa (β chain) and 138.9 kDa (α chain). The protein migrates as 70 kDa, 150 kDa and >180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

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Expression System	HEK293
Target	Complement C5
Reactivity	Human
Tag	C-Fc
Molecular Weight	73.9 kDa (β chain) & 138.9 kDa (α chain)
Expression Region	Gln 19 - Arg 677 & Thr 678 - Cys 1676
Purity	90%
Endotoxins	1.0 EU per μg
Conjugation	Unconjugated

Storage & Handling

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Storage	-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/Appearance	Powder
Buffer/Preservatives	PBS, pH 7.4
Disclaimer	For research use only

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Immobilized Human Complement C5 Protein, Fc Tag (Cat. No. orb1806482) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human C5a Human Antibody, Human IgG1 with a linear range of 0.5-4 ng/mL (QC tested).

Quick Database Links

Gene Symbol

Complement C5

UniProt

P01031

UniProt Details

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No UniProt data available

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