Human Complement C5 Protein, His-Avi Tag (Biotin)

SKU: orb1496162

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Description

Biotinylated Human Complement C5, His, is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Cys 1676 (Accession # P01031-1).

Research Area

MABSol Biotin Labeled Proteins

Images & Validation

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Application Notes

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (). The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.9 kDa (β chain) and 116.1 kDa (α chain). The protein migrates as 75 kDa, 120 kDa and 200 kDa under reducing (R) condition, and 200 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Key Properties

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Expression System	HEK293
Target	Complement C5
Reactivity	Human
Tag	C-10×His & C-Avi
Molecular Weight	73.9 kDa (β chain) & 116.1 kDa (α chain)
Expression Region	Gln 19 - Arg 677 & Thr 678 - Cys 1676
Purity	95%
Conjugation	Biotin

Storage & Handling

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Storage	-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/Appearance	Powder
Buffer/Preservatives	PBS, pH 7.4
Disclaimer	For research use only

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Human Complement C5 Protein, His-Avi Tag (Biotin)

Immobilized Biotinylated Human Complement C5, His, Avitag (Cat. No. orb1496162) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. orb668907) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human C5a Human Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (QC tested).

Quick Database Links

Gene Symbol

Complement C5

UniProt

P01031

UniProt Details

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No UniProt data available

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