Human Complement Component 5a (C5a) ELISA Kit

SKU: orb1807643

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Description

Research Area

Metabolism Research, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 1

Application Notes

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C5a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C5a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C5a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C5a. You can calculate the concentration of Human C5a in the samples by comparing the OD of the samples to the standard curve.

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, Tissue homogenate and Other biological samples
Assay Type	Sandwich
Assay Time	3.5H
Range	78.13-5000pg/mL
Sensitivity	4.63 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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C5a

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Human Complement Component 5a (C5a) ELISA Kit

Quick Database Links

Gene Symbol

C5a

UniProt

P01031

UniProt Details

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No UniProt data available

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