Human CYP2A6(Cytochrome P450 2A6) Microsample ELISA Kit

SKU: orb2997392

Description

Research Area

Immunology & Inflammation, Infectious Disease & Virology

Images & Validation

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Item 1 of 1

Application Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CYP2A6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CYP2A6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CYP2A6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CYP2A6 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	62.5-4000 pg/mL
Sensitivity	24.9 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CYP2A6; Cytochrome P450; I; Cytochrome P450; I; Cytochrome P450 IIA3; CYPIIA6; Coumarin 7-hydroxylase; CYP2A3

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Human CYP2A6(Cytochrome P450 2A6) Microsample ELISA Kit

Quick Database Links

UniProt

P11509

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No UniProt data available

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