Human CYR61(Cysteine Rich Protein, Angiogenic Inducer 61) Microsample ELISA Kit

SKU: orb2998511

Description

Images & Validation

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Application Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CYR61. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CYR61. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CYR61, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CYR61 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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Sample Types	serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	62.5-4000 pg/mL
Sensitivity	24.9 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CCN1; GIG1; IGFBP10; CCN family member 1; Insulin-like growth factor-binding protein 10

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UniProt

O00622

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No UniProt data available

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