Human DDR1(Epithelial Discoidin Domain Containing Receptor 1) ELISA Kit

SKU: orb3126863

Description

Research Area

Cardiovascular Research, Immunology & Inflammation, Metabolism Research, Signal Transduction

Images & Validation

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Item 1 of 1

Application Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DDR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DDR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DDR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DDR1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.061 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Epithelial discoidin domain receptor 1;CD167 antigen-like family member A;Cell adhesion kinase;Discoidin receptor tyrosine kinase;HGK2;CD167a;DDR1;Mammary carcinoma kinase 10;

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Human DDR1(Epithelial Discoidin Domain Containing Receptor 1) ELISA Kit

Quick Database Links

UniProt

Q08345

UniProt Details

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No UniProt data available

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