Human Decoy Receptor 3 (DcR3) ELISA Kit

SKU: orb1291332

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Description

Human DcR3 (Decoy receptor 3) ELISA Kit

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Tested Applications

ELISA

Application Notes

standard: 4000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DcR3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DcR3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DcR3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DcR3 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	62.5-4000 pg/mL
Sensitivity	25.6 pg/mL
Concentration	4000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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DcR3, Decoy receptor 3, Decoy receptor for Fas ligand, DJ583P15.1.1, M68, M68E, OTTHUMP00000031583, TNF6B_HUMAN, TNFRSF6B, TR6, Tumor necrosis factor receptor superfamily member 6B, Tumor necrosis factor receptor superfamily member 6b decoy, UNQ186/PRO212,

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UniProt

O95407

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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