Human ENO1 Protein, His Tag (HPLC verified)

SKU: orb3158140

Description

Enolase 1 (ENO1), also known as alpha‑enolase, is a glycolytic enzyme that catalyzes the conversion of 2‑phosphoglycerate to phosphoenolpyruvate. It is ubiquitously expressed and accounts for the majority of cytosolic enolase activity. Beyond its metabolic role, ENO1 is a multifunctional “moonlighting” protein: on the cell surface it acts as a plasminogen receptor promoting extracellular matrix degradation and tumor invasion, while a nuclear isoform (MBP‑1) binds the c‑MYC promoter and regulates transcription. Overexpression of ENO1 has been linked to cancer progression, autoimmune diseases, and neurodegeneration, making it both a biomarker and a potential therapeutic target.

Images & Validation

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Application Notes	This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 49.1 kDa. The protein migrates as 50-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

Key Properties

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Expression System	E. coli
Target	ENO1
Reactivity	Human
Tag	N-10×His
Molecular Weight	49.1 kDa
Expression Region	Ser 2 - Lys 434
Purity	95%
Endotoxins	1.0 EU per μg
Conjugation	Unconjugated

Storage & Handling

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Storage	-70°C
Form/Appearance	Liquid
Buffer/Preservatives	20 mM Tris, 150 mM NaCl, pH 8.0
Expiration Date	6 months from date of receipt.
Dry Ice Shipping	Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
Disclaimer	For research use only

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Quick Database Links

Gene Symbol

ENO1

RefSeq:P06733

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	P06733

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