Human Excitatory Amino Acid Transporter 3 (EAAT3) ELISA Kit

SKU: orb778293

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Excitatory Amino Acid Transporter 3(EAAT3).

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EAAT3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EAAT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EAAT3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EAAT3 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.119 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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SLC1A1 ELISA Kit, EAAC1 ELISA Kit, Solute Carrier 1 Member 1 ELISA Kit, Sodium-dependent glutamate/aspartate transporter 3 ELISA Kit, Excitatory amino-acid carrier 1 ELISA Kit, Neuronal and epithelial glutamate transporter ELISA Kit

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Human Excitatory Amino Acid Transporter 3 (EAAT3) ELISA Kit

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UniProt

P43005

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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