Human FGF1 protein (Active)

SKU: orb359150

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Description

This Human FGF1 protein (Active) spans the amino acid sequence from region 16-155aa. Purity: > 95% as determined by SDS-PAGE and HPLC.

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Application Notes	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Key Properties

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Source	E.Coli
Biological Origin	Homo sapiens (Human)
Biological Activity	Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.5 ng/ml, corresponding to a specific activity of ＞ 2.0 × 106 IU/mg in the presence of 10 μg/ml of heparin.
Tag	Tag-Free
Molecular Weight	16.0 kDa
Expression Region	16-155aa
Protein Length	Full Length of Mature Protein
Protein Sequence	M+FNLPPGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD
Purity	> 95% as determined by SDS-PAGE and HPLC.
Endotoxins	Less than 1.0 EU/µg as determined by LAL method.

Storage & Handling

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Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Form/Appearance	Lyophilized powder
Buffer/Preservatives	Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Disclaimer	For research use only

Alternative Names

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FGF-1, aFGF, Endothelial cell growth factor, ECGF, Heparin-binding growth factor 1

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SDS-PAGE analysis of Human FGF1 protein (Active)

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UniProt

P05230

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No UniProt data available

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