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Human FGFb
SKU: orb3002364
Description
Images & Validation
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Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Activity | BLI: Loaded Human FGFb (155AA) (Cat#orb3002364) on APS Biosensor, can bind Human GPC3-Fc(Cat#orb2993899) with an affinity constant of 0.68 nM as determined in BLI assay. (Regularly tested). Cell Based Assay: Measured in a cell proliferation assay using Balb/3T3 mouse fibroblast cells. The ED50 for this effect is 0.1-0.6 ng/ml. (QC verified) |
| Tag | Tag free |
| Molecular Weight | Predicted: 17.3 KDa. Observed: 17 KDa, reducing conditions |
| Expression Region | Met134-Ser288 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 8% Trehalose, 2% Mannitol, 0.05% Tween80, 2mM EDTA, pH 8.0. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; Bfgf; Heparin-binding growth factor 2; HBGF-2; FGF2; FGFB
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IF, IHC, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
30 μl, 50 μl, 100 μl, 200 μl
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Human FGFb (orb3002364)
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