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Description
Research Area
Cardiovascular Research
Images & Validation
−
Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Activity | BLI: Loaded Human FGFb (157AA) (Cat#orb3002481) on APS Biosensor, can bind Human GPC3-Fc(Cat#orb2993899) with an affinity constant of 0.7 nM as determined in BLI assay. (Regularly tested). Cell Based Assay: Measured in a cell proliferation assay using BALB/c 3T3 cells.The ED50 for this effect is 0.42 ng/ml(Regularly tested). |
| Tag | Tag free |
| Expression Region | Gly132-Ser288 |
| Endotoxins | < 0.01 EU/µg as determined by LAL test. |
| MW | Predicted: 17.4 KDa. Observed: 16 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (Regularly tested) |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, 3% Trehalose, 4% Mannitol, pH 7.5 |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; FGF2; FGFB
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Protein Handling and Storage Guide
Protein Handling Guide
Recombinant Human FGFb Protein (orb3002481)
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