Human G Protein Coupled Estrogen Receptor 1 (GPER) ELISA Kit

SKU: orb777796

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Coupled Estrogen Receptor 1(GPER).

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GPER. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GPER. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GPER, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GPER in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.123 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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GPR30 ELISA Kit, CMKRL2 ELISA Kit, DRY12 ELISA Kit, FEG-1 ELISA Kit, GPCR-Br ELISA Kit, LERGU ELISA Kit, LyGPR ELISA Kit, Membrane estrogen receptor ELISA Kit, Chemoattractant receptor-like 2 ELISA Kit, Flow-induced endothelial G-protein coupled receptor 1 ELISA Kit

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Human G Protein Coupled Estrogen Receptor 1 (GPER) ELISA Kit

Quick Database Links

UniProt

Q99527

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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