Human Gastric Intrinsic Factor (GIF) ELISA Kit

SKU: orb777076

Featured

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Gastric Intrinsic Factor(GIF) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Gastric Intrinsic Factor(GIF).

Research Area

Metabolism Research

Images & Validation

−

Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 200 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GIF protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GIF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GIF in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

−

Reactivity	Human
Sample Types	Serum, plasma and other biological fluids
Assay Type	Competitive
Assay Time	2h
Range	3.13-200 ng/mL
Sensitivity	0.91 ng/mL
Concentration	200 ng/mL

Storage & Handling

−

Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

−

TCN3 ELISA Kit, IF ELISA Kit, IFMH ELISA Kit, INF ELISA Kit, Transcobalamin III ELISA Kit, Vitamin B Synthesis ELISA Kit

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Human Gastric Intrinsic Factor (GIF) ELISA Kit

Quick Database Links

UniProt

P27352

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Manual

Product Manual

Download

Request a Document

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols