Human Heat Shock 70kDa Binding Protein 1 (HSPBP1) ELISA Kit

SKU: orb777655

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Binding Protein 1(HSPBP1).

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSPBP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSPBP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HSPBP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HSPBP1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.121 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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FES1 ELISA Kit, Hsp70 Interacting Protein ELISA Kit, Hsp70 Binding Protein,Cytoplasmic Cochaperone 1 ELISA Kit, Heat shock protein-binding protein 1 ELISA Kit, Hsp70-interacting protein 1 ELISA Kit

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Human Heat Shock 70kDa Binding Protein 1 (HSPBP1) ELISA Kit

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UniProt

Q9NZL4

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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