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Human Hemoglobin Delta (HBd) ELISA Kit

SKU: orb779194
Free Kit Promotion

Description

The Human Hemoglobin Delta (HBd) ELISA Kit is designed to quantify Hemoglobin Delta (HBd) in human serum, plasma, erythrocyte lysates and other biological fluids. The kit has a detection range of 3.13-200 µg/mL and a sensitivity of 0.91 µg/mL.

Research Area

Hematology

Images & Validation

Tested ApplicationsELISA
Application Notes
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HBd. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HBd. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBd in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

ReactivityHuman
Sample TypesSerum, plasma, erythrocyte lysates and other biological fluids
Assay TypeCompetitive
Assay Time2h
Range3.13-200 µg/mL
Sensitivity0.91 µg/mL
Concentration200 µg/mL

Storage & Handling

StorageRefer to the Storage Guidelines in the Manual
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Delta-globin ELISA Kit

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UniProt Details

No UniProt data available

Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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48 T
$ 350.00
96 T
$ 490.00
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