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Human HGF Protein, His Tag

SKU: orb700640
ActiveBiologically Active

Description

Human HGF, His Tag, premium grade is expressed from human 293 cells (HEK293). It contains AA Gln 32 - Ser 728 (Accession # P14210-1).

Research Area

Cancer Biology

Images & Validation

Application Notes
This protein carries a polyhistidine tag at the C-terminus. The mature form of HGF is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. The protein has a calculated MW of 81.6 kDa (α chain 53.7 kDa and β chain 27.9 kDa). The protein migrates as 80-90 kDa (alpha & Beta chain), 58-63 kDa (alpha chain), 33 kDa (Beta chain) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

Expression SystemHEK293
TargetHGF
ReactivityHuman
TagC-10×His
Expression RegionGln 32 - Ser 728
Endotoxins0.01 EU per μg
ConjugationUnconjugated
MW81.6 kDa (53.7 kDa|27.9 kDa)
Purity90%

Storage & Handling

Storage-20°C
Form/AppearancePowder
Buffer/PreservativesPBS, pH 7.4
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqP14210

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide