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Human IFN-alpha 1 Protein, Fc Tag
Description
Images & Validation
−| Application Notes |
|---|
Key Properties
−| Expression System | HEK293 |
|---|---|
| Target | IFN-alpha 1 |
| Reactivity | Human |
| Tag | C-Fc |
| Molecular Weight | 45.8 kDa |
| Expression Region | Cys 24 - Glu 189 |
| Purity | 95% |
| Endotoxins | 1.0 EU per μg |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
|---|---|
| Form/Appearance | Powder |
| Buffer/Preservatives | 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH 7.5 |
| Disclaimer | For research use only |
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 73 kDa after removal of the signal peptide. The apparent molecular mass of IFNAR1-mFc is approximately 100-130 kDa due to glycosylation.
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50 μg, 10 μg, 100 μgHuman IFNA1 Protein, hFc Tag [orb1290870]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 45.5 kDa after removal of the signal peptide. The apparent molecular mass of IFNA1-hFc is approximately 35-55 kDa due to glycosylation.
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Loaded Human IFN-alpha / beta R1, His Tag (Cat. No. orb867302) on NTA Biosensor, can bind Human IFN-alpha 1, Fc Tag (Cat. No. orb1147003) with an affinity constant of 11.3 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
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Protocol Information
Human IFN-alpha 1 Protein, Fc Tag (orb1147003)
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