Recombinant Human IFN alpha2b Protein

SKU: orb3002431

Description

Recombinant Human Interferon Alpha-2b is produced by our E.coli expression system and the target gene encoding Cys24-Glu188 is expressed.

Images & Validation

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Key Properties

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Expression System	E.coli
Biological Activity	BLI: Loaded Human IFNAR2-Fc(Cat#orb2994011) on Protein A Biosensor, can bind Human IFN alpha2b(Cat#orb3002431) with an affinity constant of 2.98 nM as determined in BLI assay. (Regularly tested). Cell Based Assay: Measured in antiviral assay using A549 human lung cancer cells infected with vesicular stomatitisvirus (VSV) The ED50 for this effect is 5 ng/mL.
Tag	Tag free
Molecular Weight	Predicted: 19.4 KDa. Observed: 17 KDa, reducing conditions
Expression Region	Cys24-Glu188
Purity	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified). SEC-HPLC: Greater than 90% as determined by SEC-HPLC. (QC verified)
Endotoxins	< 1 EU/µg as determined by LAL test.

Storage & Handling

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Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Interferon Alpha-2; IFN-Alpha-2; Interferon Alpha-A; LeIF A; IFNA2

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Quick Database Links

UniProt

P01563

UniProt Details

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No UniProt data available

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