You have no items in your shopping cart.
Human IgG (H&L) Antibody Peroxidase Conjugated
SKU: orb347290
Description
Images & Validation
−Item 1 of 2
| Tested Applications | DOT, ELISA, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:150,000 - 1:250,000, IHC: 1:1,000 - 1:5,000, WB: 1:5,000 - 1:25,000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-Human IgG whole molecule was produced by repeated immunization with Human IgG whole molecule in rabbit. |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgG and Human Serum. |
| Conjugation | HRP |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Concentration | 2.0 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-Human IgG Antibody peroxidase Conjugation, rabbit anti-Human IgG HRP Conjugated Antibody
Similar Products
−- Item 1 of 3
Human IgG (H&L) Antibody Peroxidase Conjugated [orb346660]
ELISA, IHC, WB
Human
Rabbit
Polyclonal
HRP
20 mg - Item 1 of 2
Human IgG (H&L) Antibody Peroxidase Conjugated [orb347222]
DOT, ELISA, IHC, WB
Human
Goat
Polyclonal
HRP
2 mg - Item 1 of 2
Human IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed [orb347230]
ELISA, IHC, WB
Human
Goat
Polyclonal
HRP
2 mg - Item 1 of 2
Human IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed [orb347231]
ELISA, IHC, WB
Human
Goat
Polyclonal
HRP
100 μg - Item 1 of 1
Human IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-Adsorbed [orb347192]
ELISA, IHC, WB
Human
Goat
Polyclonal
HRP
1 mg
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
Dot Blot Results of Rabbit Anti-Human IgG HRP Conjugated Antibody. Human IgG sample loaded 1- 100 ng, 2- 33.3 ng, 3- 11.1 ng, 4- 3.7 ng, 5- 1.2 ng. Rabbit Anti-Human IgG Peroxidase Conjugated Antibody 1:1000 for 60 mins at RT. Blocking Buffer for Fluorescent Western Blotting (p/n orb348637) for 1hr at RT.
ELISA results of Rabbit anti-Human IgG Antibody Peroxidase Conjugated tested against purified Human IgG protein. Each well was coated in duplicate with 1.0 µg of Human IgG (p/n orb346219). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate p/n orb348651.
Quick Database Links
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
DOT
Dot Blot
Human IgG (H&L) Antibody Peroxidase Conjugated (orb347290)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Usually dispatched within 5-10 working days