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Human IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
SKU: orb347195
Description
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| Tested Applications | DOT, ELISA, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:10,000 - 1:30,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Human IgG whole molecule |
| Purity | Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins. |
| Conjugation | AP |
Storage & Handling
−| Storage | Store secondary antibody at 4° C before opening. DO NOT FREEZE. Alkaline Phosphatase Conjugated Secondary Antibodies are stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.1% (w/v) Sodium Azide |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−goat anti-Human IgG Alkaline Phosphatase Conjugation, goat anti-Human IgG ALP conjugate, goat anti-human IgG Alk Phos conjugated antibody
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ELISA Results of Goat Anti-Human IgG Antibody Alkaline Phosphatase Conjugated (Min X Bv Ch Gt GP Ham Hs Ms Rb Rt Sh Serum Proteins) tested against purified Human IgG Alk Phos. Each well was coated in duplicate with 1.0 µg of Human IgG (p/n orb346219). The working dilution is 1:19600. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using ELISA Alkaline Phosphatase Substrate Buffer and NPP Working Buffer.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
DOT
Dot Blot
Human IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed (orb347195)
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