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Human IL-1 beta Protein
Description
Images & Validation
−| Application Notes |
|---|
Key Properties
−| Expression System | E. coli |
|---|---|
| Target | IL-1 beta |
| Reactivity | Human |
| Tag | Native |
| Molecular Weight | 17.5 kDa |
| Expression Region | Ala 117 - Ser 269 |
| Purity | 95% |
| Endotoxins | 0.01 EU per μg |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
|---|---|
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Disclaimer | For research use only |
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−Human IL1B protein (Active) [orb358966]
> 97% as determined by SDS-PAGE and HPLC.
17.3 kDa
E.Coli
10 μg, 100 μg, 500 μgRecombinant Human IL1B Protein, hFc Tag [orb1173751]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 43.5 kDa after removal of the signal peptide.The apparent molecular mass of IL1B-hFc is approximately 35-55 kDa due to glycosylation.
Mammalian
10 μg, 100 μg, 50 μg

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SDS-PAGE analysis of Human IL-1 beta protein

Human IL-1 RII, Fc Tag (Cat. No. orb257592) captured on CM5 chip via anti-human IgG Fc antibodies surface can bind Human IL-1 beta, premium grade (Cat. No. orb257615) with an affinity constant of 0.622 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
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UniProt Details
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Human IL-1 beta Protein (orb257615)
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