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Recombinant Human IL1B Protein, hFc Tag
Description
Images & Validation
−Key Properties
−| Source | Mammalian |
|---|---|
| Target | IL1B |
| Reactivity | Human |
| Tag | C-Human Fc Tag |
| Molecular Weight | The protein has a predicted molecular mass of 43.5 kDa after removal of the signal peptide.The apparent molecular mass of IL1B-hFc is approximately 35-55 kDa due to glycosylation. |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
Storage & Handling
−| Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
| Disclaimer | For research use only |
Alternative Names
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Recombinant Human IL1B Protein, hFc Tag on SDS-PAGE under reducing condition

ELISA plate pre-coated by 1 μg/mL (100 μL/well) Recombinant Human IL1B Protein, hFc Tag (orb1173751) can bind IL1B Biosimilar Antibody(orb1291211) in a linear range of 0.61–156.25 ng/mL

ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human IL1R2 Protein, His Tag (orb1290929) can bind Recombinant Human IL1B Protein, hFc Tag (orb1173751) in a linear range of 3.20–400 ng/mL

ELISA plate pre-coated by 2 μg/mL (100 μL/well) Recombinant Human IL1B Protein, hFc Tag (orb1173751) can bind Human IL1RA Protein, mFc Tag (orb2321379) in a linear range of 0.64–80 ng/mL
Quick Database Links
UniProt Details
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Protocol Information
Recombinant Human IL1B Protein, hFc Tag (orb1173751)
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