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Human IL-1b
SKU: orb3002471
Description
Images & Validation
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Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Activity | Cell Based Assay: Measured by its ability to induce NF-kB signaling in 293-IL1 Res cells(Cat#Xorb3002421). The ED50 for this effect is 20-100 pg/ml. |
| Tag | Tag free |
| Molecular Weight | Predicted: 17.5 KDa. Observed: 17 KDa, reducing conditions |
| Expression Region | Ala117-Ser269 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 0.01 EU/µg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM PB，150 mM NaCl，8% Trehalose，0.02% Tween 80，4% Mannitol，pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Interleukin-1 beta; Catabolin; IL1F2; IL1B
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Human IL-1b (orb3002471)
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