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Human IL-7
SKU: orb3002429
Description
Images & Validation
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Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Activity | BLI: Loaded Human IL-7RA-His(Cat#orb2993835) on HIS1K Biosensor, can bind Human IL-7(Cat#orb3002429) with an affinity constant of 15.04 nM as determined in BLI assay. Loaded Human IL-7RA-Fc-His(Cat#orb2994563) on HIS1K Biosensor, can bind Human IL-7(Cat#orb3002429) with an affinity constant of 18.59 nM as determined in BLI assay. Loaded Mouse IL-7RA-Fc(Cat#orb2994406) on Protein A Biosensor, can bind Human IL-7(Cat#orb3002429) with an affinity constant of 29.2 nM as determined in BLI assay. . Cell Based Assay: Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). The ED50 for this effect is 0.02-0.08 ng/ml. |
| Tag | Tag free |
| Molecular Weight | Predicted: 17.5 KDa. Observed: 18 KDa, reducing conditions |
| Expression Region | Asp26-His177 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (Regularly tested) |
| Endotoxins | < 0.01 EU/µg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 300mM NaCl, pH 8.0. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−Interleukin-7; IL-7; IL7
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Human IL-7 (orb3002429)
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