IL12B/IL-12 p40/NKSF2 Human Monoclonal Antibody

SKU: orb2958448

Description

IL12B/IL-12 p40/NKSF2 Human Monoclonal Antibody is an unconjugated monoclonal antibody. It shows reactivity with Human samples. It is suitable for use in ELISA, FC and WB applications. The antibody is supplied in liquid form. It is Protein A/G purified from cell culture supernatant. Purity is >95% as determined by SDS-PAGE.

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, FC, WB
Reactivity	Human

Key Properties

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Antibody Type	Recombinant Antibody
Host	Human
Clonality	Monoclonal
Isotype	IgG1, lambda
Clone No.	BUCC4239
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin	Please contact with the lab for this information.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M PBS, pH 7.4.
Concentration	Lot dependent
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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IL-12 subunit p40, IL12B, Interleukin-12 subunit beta, IL-12B, CLMF p40, NKSF2, NK cell stimulatory factor chain 2, Cytotoxic lymphocyte maturation factor 40 kDa subunit

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IL12B/IL-12 p40/NKSF2 Human Monoclonal Antibody

Flow-cytometry using anti-human IL12B antibody. IL12B Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL12B antibody monoclonal antibody (Catalog # orb2958448, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Anti-Human IL12B/IL-12 p40/NKSF2 Antibody.

Western blot analysis was performed using anti-IL12B/IL-12 p40/NKSF2 monoclonal antibody at 1 µg/mL on various samples. Lane 1: recombinant human IL12B/IL-12 p40/NKSF2 (Catalog No: orb2968761), 50 ng, Lane 2: recombinant human IL12B/IL-12 p40/NKSF2 (Catalog No: orb2968761), 30 ng, Lane 3: recombinant human IL12B/IL-12 p40/NKSF2 (Catalog No: orb2968761), 20 ng.

Quick Database Links

UniProt

P29460

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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