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IL12B/IL-12 p40/NKSF2 Human Monoclonal Antibody

SKU: orb2958448

Description

Anti-Human IL12B/IL-12 p40/NKSF2 Antibody (BUCC4239) is a human monoclonal antibody detecting IL-12 subunit p40 in ELISA, FCM, WB. Suitable for Human. Highlights Multi-Application: Validated across multiple applications. Flow Cytometry: Validated for flow cytometry applications.

Research Area

Immunology & Inflammation

Images & Validation

Tested ApplicationsELISA, FC, WB
ReactivityHuman

Key Properties

Antibody TypeRecombinant Antibody
HostHuman
ClonalityMonoclonal
IsotypeIgG1, lambda
Clone No.BUCC4239
Purity>95% as determined by SDS-PAGE.
PurificationProtein A/G purified from cell culture supernatant.
EndotoxinPlease contact with the lab for this information.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M PBS, pH 7.4.
ConcentrationLot dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

IL-12 subunit p40, IL12B, Interleukin-12 subunit beta, IL-12B, CLMF p40, NKSF2, NK cell stimulatory factor chain 2, Cytotoxic lymphocyte maturation factor 40 kDa subunit

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IL12B/IL-12 p40/NKSF2 Human Monoclonal Antibody

Flow-cytometry using anti-human IL12B antibody. IL12B Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL12B antibody monoclonal antibody (Catalog # orb2958448, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody and cells analysed on a NovoCyte Flow Cytometer.

IL12B/IL-12 p40/NKSF2 Human Monoclonal Antibody

SDS-PAGE for Anti-Human IL12B/IL-12 p40/NKSF2 Antibody.

IL12B/IL-12 p40/NKSF2 Human Monoclonal Antibody

Western blot analysis was performed using anti-IL12B/IL-12 p40/NKSF2 monoclonal antibody at 1 µg/mL on various samples. Lane 1: recombinant human IL12B/IL-12 p40/NKSF2 (Catalog No: orb2968761), 50 ng, Lane 2: recombinant human IL12B/IL-12 p40/NKSF2 (Catalog No: orb2968761), 30 ng, Lane 3: recombinant human IL12B/IL-12 p40/NKSF2 (Catalog No: orb2968761), 20 ng.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

IL12B/IL-12 p40/NKSF2 Human Monoclonal Antibody (orb2958448)

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Available Sizes

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50 μg
$ 180.00
100 μg
$ 290.00
1 mg
$ 1,410.00
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