Human Interleukin 6 (IL6) ELISA Kit

SKU: orb775021

Featured

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 6(IL6).

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Infectious Disease & Virology

Images & Validation

−

Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL6 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

−

Reactivity	Human
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	7.82-500 pg/mL
Sensitivity	3.3 pg/mL
Concentration	500 pg/mL

Storage & Handling

−

Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

−

MGI2-A ELISA Kit, MGI2A ELISA Kit, HGF ELISA Kit, BSF2 ELISA Kit, HSF ELISA Kit, IFNB2 ELISA Kit, B-Cell Stimulatory Factor-2 ELISA Kit, Hybridoma/Plasmacytoma Growth Factor ELISA Kit, Hepatocyte Stimulating Factor ELISA Kit, Cytotoxic T-Cell Differentiation Factor ELISA Kit

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Quick Database Links

UniProt

P05231

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Manual

Product Manual

Download

Request a Document

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols

Filter by Applications

ELISA (3)

Filter by Species

Human (3)

M Świderek-Matysiak et al. A Long-Term Prospective Evaluation of the Predictive Value of Molecular Markers in Serum and Cerebrospinal Fluid in Patients in the Early Stage of Multiple Sclerosis International Journal of Molecular Sciences, (2026)

PubMed:

Applications

ELISA

Reactivity

Human

Anita Gąsiorowska 1ORCID,Marek Romanowski 1,Ewa Walecka-Kapica 1,Aleksandra Kaczka 1,Cezary Chojnacki 1ORCID,Milena Padysz 1,Marta Siedlecka 1,Julia Banasik 1,Aleksandra Sobolewska-Włodarczyk 1,Maria Wiśniewska-Jarosińska 1,Joanna B. Bierła 2ORCID,Nize Otaru 3,Bożena Cukrowska 4,*ORCID andRobert E. Steinert Robert E. Steinert SciProfilesScilitPreprints.orgGoogle Scholar Efficacy and Safety of a Mixture of Microencapsulated Sodium Butyrate, Probiotics, and Short Chain Fructooligosaccharides in Patients with Irritable Bowel Syndrome—A Randomized, Double-Blind, Placebo-Controlled Study Journal of Clinical Medicine, (2024)

PubMed:

Applications

ELISA

Reactivity

Human

Jasmin Bayer,Nicole Karoline Petersen,Jeanine Veruschka Hess,Yvonne Jockel-Schneider,Petra Högger * Impact of a Dietary Supplementation with French Maritime Pine Bark Extract Pycnogenol® on Salivary and Serum Inflammatory Biomarkers During Non-Surgical Periodontal Therapy—A Randomized Placebo-Controlled Double-Blind Trial preprints.org, (2025)

PubMed:

Applications

ELISA

Reactivity

Human