Human IL6 protein

SKU: orb391992

Description

Recombinant human IL6 protein is produced by our E.coli expression system and the target gene encoding Pro29-Met212 is expressed.

Research Area

Epigenetics, Immunology

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	E. coli
Target	IL6
Reactivity	Human
Protein Sequence	PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM
Purity	Greater than 98% as determined by RP-HPLC and reducing SDS-PAGE.
Endotoxins	Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

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Storage	Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μM filtered solution of 20mM HAc-NaAc, 150mM NaCl, pH 5.0
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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IFNB2; Interleukin-6; IL-6; B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2

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Quick Database Links

Gene Symbol

IL6

UniProt

P05231

NCBI Gene ID

3569

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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