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Description
Images & Validation
−| Tested Applications | FA, HPLC, SDS-PAGE |
|---|---|
| Application Notes |
Key Properties
−| Biological Origin | E.coli |
|---|---|
| Biological Activity | The ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is less than 0.1 ng/ml, corresponding to the specific activity of 1.0 10,000,000 Units per mg. |
| Target | Human IL6 |
| Conjugation | Unconjugated |
| Purity | > 97.0% as determined by RP-HPLC and analysis by SDS-PAGE |
| Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Pro-Val-Pro-Pro. |
Storage & Handling
−| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
|---|---|
| Buffer/Preservatives | Lyophilized from 0.2 um filtered concentrated (1mg/ml) solution in PBS, pH 7.4. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Recombinant Human Interleukin-6 (IL6) Protein (Active) [orb1881858]
Greater than 95% as determined by SDS-PAGE
22.8 kDa
Yeast
1 mg, 100 μg, 20 μgHuman CCAAT/Enhancer Binding Protein Beta (C/EBPβ) ELISA Kit [orb1949549]
Human
0.16-10ng/mL
0.1 ng/mL
48 T, 96 THuman CCAAT/Enhancer Binding Protein Beta (CEBPb) ELISA Kit [orb775897]
Human
0.16-10 ng/mL
0.061 ng/mL
96 T, 48 TRecombinant Human IL-6 Protein [orb2995250]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 20.9 KDa. Observed: 20 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg

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SDS-PAGE analysis of Human IL6 protein
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Protocol Information
Giampaolo Bosi 1, Paolo Merella 2, Emanuela Franchella 3, Bahram Sayyaf Dezfuli 4, Luisa Giari The Leydig organ of elasmobranchs: Shed light on an active and mysterious defense center with immunological characterization of its cells Tissue Cell, (2025)
Human IL6 protein (orb80063)
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