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Human IL6 protein Discontinued

SKU: orb80063

Description

Human IL6 protein

Images & Validation

Tested ApplicationsFA, HPLC, SDS-PAGE
Application Notes
Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of IL-6 as Reference Standard.

Key Properties

Biological OriginE.coli
Biological ActivityThe ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is less than 0.1 ng/ml, corresponding to the specific activity of 1.0 10,000,000 Units per mg.
TargetHuman IL6
ConjugationUnconjugated
Purity> 97.0% as determined by RP-HPLC and analysis by SDS-PAGE
Protein SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Pro-Val-Pro-Pro.

Storage & Handling

StorageStore at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Buffer/PreservativesLyophilized from 0.2 um filtered concentrated (1mg/ml) solution in PBS, pH 7.4.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Interleukin BSF 2 protein, B cell differentiation factor protein, B cell stimulatory factor 2 protein, BSF 2 protein, BSF-2 protein, BSF2 protein, CDF protein, CTL differentiation factor protein, Cytotoxic T cell differentiation factor protein, HGF protein, HPGF protein, HSF protein, Hybridoma growth factor protein, Hybridoma plasmacytoma growth factor protein, IFNB2 protein, IL 6 protein, IL-6 protein, IL6 protein, IL6 protein protein, Interleukin 6 protein, Interleukin 6 protein, Interleukin BSF 2 protein, Interleukin-6 protein, Interleukin6 protein

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Human IL6 protein

SDS-PAGE analysis of Human IL6 protein

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Protocol Information

SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
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HPLC
High-Performance Liquid Chromatography
View Protocol

Giampaolo Bosi 1, Paolo Merella 2, Emanuela Franchella 3, Bahram Sayyaf Dezfuli 4, Luisa Giari The Leydig organ of elasmobranchs: Shed light on an active and mysterious defense center with immunological characterization of its cells Tissue Cell, (2025)

Human IL6 protein (orb80063)

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