Human Interleukin 18 Receptor Accessory Protein (IL18RAP) ELISA Kit

SKU: orb777337

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 18 Receptor Accessory Protein(IL18RAP).

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Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL18RAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL18RAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL18RAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL18RAP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.053 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CDw218b ELISA Kit, CD218-B ELISA Kit, IL-1R7 ELISA Kit, ACPL ELISA Kit, Accessory protein-likeL ELISA Kit, CD218 antigen-like family member B ELISA Kit, IL-1R accessory protein-like ELISA Kit, Interleukin-1 receptor 7 ELISA Kit, Interleukin-18 receptor beta ELISA Kit

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Human Interleukin 18 Receptor Accessory Protein (IL18RAP) ELISA Kit

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UniProt

O95256

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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