Human Interleukin 2 Receptor Gamma (IL2Rg) ELISA Kit

SKU: orb778562

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 2 Receptor Gamma(IL2Rg).

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Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL2Rg. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL2Rg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL2Rg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL2Rg in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.055 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CD132 ELISA Kit, IL2R-g ELISA Kit, IL2-RG ELISA Kit, CD132 ELISA Kit, IMD4 ELISA Kit, p64 ELISA Kit, SCIDX ELISA Kit, SCIDX1 ELISA Kit, Cytokine Receptor Common Gamma Chain ELISA Kit, Severe Combined Immunodeficiency ELISA Kit

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Human Interleukin 2 Receptor Gamma (IL2Rg) ELISA Kit

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UniProt

P31785

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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