Human KLK7 protein

SKU: orb392040

Description

Recombinant human KLK7 protein is produced by our Mammalian expression system and the target gene encoding Glu23-His252 is expressed with a 6His tag at the C-terminus.

Research Area

Epigenetics

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	Human cells
Target	KLK7
Reactivity	Human
Protein Sequence	EEAQGDKIID GAPCARGSHP WQVALLSGNQ LHCGGVLVNE RWVLTAAHCK MNEYTVHLGS DTLGDRRAQR IKASKSFRHP GYSTQTHVND LMLVKLNSQA RLSSMVKKVR LPSRCEPPGT TCTVSGWGTT TSPDVTFPSD LMCVDVKLIS PQDCTKVYKD LLENSMLCAG IPDSKKNACN GDSGGPLVCR GTLQGLVSWG TFPWGQPNDP GVYTQVCKFT KWINDTMKKH VDHHHHHH
Purity	Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Endotoxins	Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

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Storage	Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.2 μM filtered solution of 20mM HEPES, 150mM NaCl, pH 7.5.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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PRSS6; SCCE; Kallikrein-7; hK7; Serine protease 6; Stratum corneum chymotryptic enzyme; hSCCE

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Quick Database Links

Gene Symbol

KLK7

UniProt

P49862

NCBI Gene ID

5650

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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