Human λ-IgLC (lambda immunoglobulin light chain) ELISA Kit

SKU: orb2937732

Description

Research Area

Immunology & Inflammation

Images & Validation

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Tested Applications

ELISA

Application Notes

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human λ-IgLC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human λ-IgLC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human λ-IgLC in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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Reactivity	Human
Sample Types	serum, plasma and other biological fluids
Assay Type	Competitive
Assay Time	2h
Range	0.32-20 µg/mL
Sensitivity	0.12 µg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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lambda immunoglobulin light chain, lambda-IgLC

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Quick Database Links

Gene Symbol

λ-IgLC

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Datasheet

Product Information

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Manual

Product Manual

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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