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Recombinant Human LIF Protein
Description
Images & Validation
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Key Properties
−| Expression System | E.coli |
|---|---|
| Tag | Tag free |
| Molecular Weight | Predicted: 19.7 KDa. Observed: 18 KDa, reducing conditions |
| Expression Region | Ser23-Phe202 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 0.01 EU/µg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM PB,2% Sucrose,4% Mannitol,0.02% Tween 80,pH7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−LIF (NM_002309) Human Recombinant Protein [orb3035936]
>95% as determined by SDS-PAGE and Coomassie blue staining
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 46 kDa after removal of the signal peptide. The apparent molecular mass of LIF-His is approximately 55-70 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μgRecombinant Human CD118/LIFR Protein, C-His [orb2966401]
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95.72 kDa
1 mg, 50 μg, 100 μgRecombinant Human CD118/LIFR Protein, N-His [orb2968547]
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>90% as determined by SDS-PAGE.
18.17 kDa
1 mg, 50 μg, 100 μg

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UniProt Details
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Protocol Information
Recombinant Human LIF Protein (orb3002454)
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