Human LIF protein (Active)

SKU: orb359146

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Active

Description

This Human LIF protein (Active) spans the amino acid sequence from region 23-202aa. Purity: > 98% as determined by SDS-PAGE and HPLC.

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 1

Application Notes

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Key Properties

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Source	E.Coli
Biological Origin	Homo sapiens (Human)
Biological Activity	Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of ＞ 1.0 x 10 ^ 7 IU/mg.
Tag	Tag-Free
Expression Region	23-202aa
Protein Length	Full Length of Mature Protein
Endotoxins	Less than 1.0 EU/μg as determined by LAL method.
MW	19.7 kDa
Purity	> 98% as determined by SDS-PAGE and HPLC.
Protein Sequence	SPLPITPVNATCAIRHPCHNNLMNQIRSQLAQLNGSANALFILYYTAQGEPFPNNLDKLCGPNVTDFPPFHANGTEKAKLVELYRIVVYLGTSLGNITRDQKILNPSALSLHSKLNATADILRGLLSNVLCRLCSKYHVGHVDVTYGPDTSGKDVFQKKKLGCQLLGKYKQIIAVLAQAF

Storage & Handling

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Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Form/Appearance	Lyophilized powder
Buffer/Preservatives	Lyophilized from a 0.2 μm filtered PBS, pH 7.4
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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LIF, D factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin

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SDS-PAGE gel analysis of orb359146.

Quick Database Links

UniProt

P15018

UniProt Details

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No UniProt data available

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