Human Lipopolysaccharide (LPS) ELISA Kit

SKU: orb782855

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipopolysaccharide(LPS).

Research Area

Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 3000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LPS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LPS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LPS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LPS in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	46.88-3000 pg/mL
Sensitivity	14.6 pg/mL
Concentration	3000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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LOS ELISA Kit, Lipoglycans ELISA Kit, Lipooligosaccharide ELISA Kit, Lipo-Oligosaccharide ELISA Kit, Endotoxin ELISA Kit

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Human Lipopolysaccharide (LPS) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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