Human / Mouse / Rat Histone H2B (H2B) ELISA Kit

SKU: orb778744

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone H2B(H2B).

Research Area

Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human,Rat,Mouse H2B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human,Rat,Mouse H2B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human,Rat,Mouse H2B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human,Rat,Mouse H2B in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human, Mouse, Rat
Sample Types	tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	3.13-200 ng/mL
Sensitivity	1.15 ng/mL
Concentration	200 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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HIST1H2BA, testis, bA317E16.3, H2B, H2B histone family member U, H2B histone family member U testis specific, H2B histone family, member U, (testis specific), H2B testis, H2B1A_HUMAN, H2BFU, H2BT, HIST1H2BA, Histone 1, H2ba, Histone cluster 1 H2ba, Histone H2B, Histone H2B testis, Histone H2B type 1 A, Histone H2B type 1-A, Histone H2B type 1A, STBP, Testis specific histone H2B, Testis-specific histone H2B, TSH 2B, TSH2B, TSH2B.1,

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Human / Mouse / Rat Histone H2B (H2B) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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