Human Myeloid Progenitor Inhibitory Factor 1 (MPIF1) ELISA Kit

SKU: orb776339

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myeloid Progenitor Inhibitory Factor 1(MPIF1).

Research Area

Cancer Biology, Immunology & Inflammation, Infectious Disease & Virology

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MPIF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MPIF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MPIF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MPIF1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	15.63-1000 pg/mL
Sensitivity	0.49 pg/mL
Concentration	1000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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CCL23 ELISA Kit, SCYA23 ELISA Kit, Ckb-8 ELISA Kit, MIP-3 ELISA Kit, CKb8 ELISA Kit, Chemokine(C-C-Motif)ligand 23 ELISA Kit, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 23 ELISA Kit, Macrophage inflammatory protein 3 ELISA Kit

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Human Myeloid Progenitor Inhibitory Factor 1 (MPIF1) ELISA Kit

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UniProt

P55773

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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